Nicking Saturation Mutagenesis - a Fast and Kit-Compatible Method to Create Mutational Libraries

 

Executive Summary

 

There is a huge interest in the research and pharmaceutical industry to improve protein properties by mutation. Enzyme and antibody engineering have become key areas of worldwide R&D activity in the private and university sector. The demand for simple, cheap, fast, and robust methods to prepare comprehensive mutagenesis libraries is enormous. Nicking Saturation Mutagenesis (NSM), a novel method recently developed at Michigan State University, meets all of these requirements. 

 

Description of Technology

 

Researchers at MSU have developed a new method, Nicking Saturation Mutagenesis (as published in Nature Method),  that allows protein engineering companies and academic research labs to cost-effectively and precisely create their own user-defined, comprehensive mutational library in only one single day. This plasmid-based, single-pot saturation mutagenesis is kit-compatible and very easy to handle, while being extremely effective and producing results of the highest quality. It enables researchers to access all possible single point mutations in a given DNA sequence. NSM is a very efficient method that significantly reduces labor and material cost.

 

Key Benefits

  • Kit-compatible – The technology is kit-compatible and very easy to handle, all steps of the method are performed in one single test tube.
  • Fast and scalable – Protein engineering companies and academic labs can create their own user-defined mutational libraries in a single day.
  • Low cost – The technology provides premium quality results at a scalable quantity in a much more cost-effective and less complicated, less labor-intensive way than all other methods currently available on the market.
  • Effective and precise – The effectiveness & precision of Nicking Saturation Mutagenesis have been experimentally validated on multiple systems.
  • Comprehensive – Access to all possible single point mutations in a given DNA sequence.

 

Applications

  • Protein engineering
  • R&D
  • Creation of mutational libraries

 

Patent Status: 

 

Patent pending

 

Publication:

 

http://www.nature.com/nmeth/journal/v13/n11/full/nmeth.4029.html?foxtrotcallback=true/u

 

Licensing Rights Available

 

Full licensing rights available

 

Inventors: Timothy Whitehead, Emily Wrenbeck, Justin Klesmith, James Stapleton

 

Tech ID: TEC2016-0133

 

 

Patent Information:

Category(s):

For Information, Contact:

Anne DiSante
Associate Director
Michigan State University
disante@msu.edu
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