CH12F3 Cell Line with Various Knock-Outs

 

Executive Summary

 

The CH12F3 mouse B lymphocyte cell lines are useful tools to study the role of DNA metabolism in modulating immunoglobulin genes and DNA repair. Since enzymes involved in DNA metabolism play essential roles in embryogenesis, knockout of these proteins often resulted in lethality in mouse embryonic stem cells. MSU researchers have developed various enzyme-deficient CH12F3 cell lines compatible with cell viability. Key enzymes involved in DNA repair processes, such as ligases, endonucleases, deaminases and more, have been targeted for selective knockouts.

 

Description of Technology

 

Antigen-stimulated mature B cells can diversify their immunoglobulin gene by class switch recombination (CSR), somatic hypermutation and gene conversion. A diversification of the constant region of the Ig heavy chain in CSR allows a “switch” of the class (or isotype) of the expressed immunoglobulin, without altering its antigen specificity.  Apyrimidinic endonucleases (APE) generate DNA strand incisions, activation-induced cytidine deaminase (AID) and DNA Ligases, which repair DNA double strand breaks (DSB), non-homologous end joint (NHEJ) and alternative end-joining (A-EJ) pathways have been targeted. Additional proteins involved in single strand DNA repairs and others are listed and are currently being developed. CRISPR/Cas9 technology has been used and recombination-mediated cassette exchange (RMCM) allows repeated knock-ins at the same genomic locus.

 

Key Benefits

  • Immunology Research
  • Study of enzymes in DNA repair processes

 

Applications

  • Extensive new research opportunities

 

Patent Status:

 

Proprietary materials

 

Licensing Rights Available

 

Non-exclusive license rights are available. 

 

Inventors: Kefei Yu

Tech ID: TEC2016-0053 to 0067

 

 

Patent Information:

Category(s):

For Information, Contact:

Randy Ramharack
Technology Manager
Michigan State University
ramharac@msu.edu
Inventors:
Kefei Yu
Keywords: