MSU Technologies

Nicking Saturation Mutagenesis - a Fast and Kit-Compatible Method to Create Mutational Libraries

Executive Summary

There is a huge interest in the research and pharmaceutical industry to improve protein properties by mutation. Enzyme and antibody engineering have become key areas of worldwide R&D activity in the private and university sector. The demand for simple, cheap, fast, and robust methods to prepare comprehensive mutagenesis libraries is enormous. Nicking Saturation Mutagenesis (NSM), a novel method recently developed at Michigan State University, meets all of these requirements.

Description of Technology

Researchers at MSU have developed a new method, Nicking Saturation Mutagenesis (as published in Nature Method), that allows protein engineering companies and academic research labs to cost-effectively and precisely create their own user-defined, comprehensive mutational library in only one single day. This plasmid-based, single-pot saturation mutagenesis is kit-compatible and very easy to handle, while being extremely effective and producing results of the highest quality. It enables researchers to access all possible single point mutations in a given DNA sequence. NSM is a very efficient method that significantly reduces labor and material cost.

Key Benefits

Kit-compatible – The technology is kit-compatible and very easy to handle, all steps of the method are performed in one single test tube.
Fast and scalable – Protein engineering companies and academic labs can create their own user-defined mutational libraries in a single day.
Low cost – The technology provides premium quality results at a scalable quantity in a much more cost-effective and less complicated, less labor-intensive way than all other methods currently available on the market.
Effective and precise – The effectiveness & precision of Nicking Saturation Mutagenesis have been experimentally validated on multiple systems.
Comprehensive – Access to all possible single point mutations in a given DNA sequence.

Applications