High Throughput Production of Recombinant Adeno-Associated Virus Libraries

 

Executive Summary

 

Each year the number of published genomes increases, challenging scientists to identify the purpose of thousands of new genes of unknown function.  Analysis of such a large volume of data calls for high throughput screening of genetic material, which plays a key role in modern drug discovery.  The use of viral vectors to perform high throughput genomic work has been limited to retroviruses or lentiviruses to deliver libraries of genetic material.  MSU inventors have developed a novel high throughput method for screening libraries using recombinant adeno-associated virus (rAAV) which expands compatibility for high throughput screens to cell types that are refractory to lenti- and retroviruses.

 

Description of Technology

 

rAAV is capable of transducing cell types that lentivirus and retrovirus cannot, such as T-cells, B-cells, monocytes, and dendritic cells.  The screens with rAAV libraries will allow for pharmaceutical companies to investigate potential therapies in these cell types in a robust manner.  The method can be used with robotics to screen upwards of 20,000 viral clones simultaneously, allowing for extremely high throughput library evaluation.

 

Key Benefits

  • Therapeutic development – high throughput functional screening for identifying new therapeutic targets
  • Expand high throughput screening applications – rAAV vectors would expand high throughput genomic applications to cell types such as T-cells, B-cells, monocytes, and dendritic cells.

 

Applications

  • Genetic library screens for libraries exceeding 20,000 clones

 

Patent Status: 

 

Under review.

 

Licensing Rights Available

 

Full licensing rights available.

 

Inventors: Fredric Manfredsson

 

Tech ID: TEC2015-0024

 

Alternative contact due to temporary leave:

 

Nina (Isi) Davis, Technology Marketing Manager, email: davisnin@msu.edu, phone (direct): (517)884-1829. 

 

Patent Information:

Category(s):

For Information, Contact:

Randy Ramharack
Technology Manager
Michigan State University
ramharac@msu.edu
Inventors:
Fredric Manfredsson
Keywords: